Improvement of xylanase production by Cochliobolus sativus in solid state fermentation

نویسندگان

  • Yasser Bakri
  • Mohammed Jawhar
  • Mohammed Imad Eddin Arabi
چکیده

The xylanase production by Cochliobolus sativus strain Cs6 was improved under solid state fermentation (SSF). High xylanase activity (1079 U/g) was obtained when wheat straw was used after 8 days of incubation. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production (1543 and 1483 U/g, respectively). The Cs6 strain grown in SSF in a simple medium, proved to be a promising microorganism for xylanase production.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Improvement of Xylanase Production by Cochliobolus sativus in Submerged Culture

The xylanase production by a new Cochliobolus sativus Cs5 strain was improved under submerged fermentation. The xylanase was induced by xylan and repressed by glucose, sucrose, maltose, xylose, starch and cellulose. Highest enzyme production (98.25 IU/mL) was recorded when wheat straw (4 % by mass per volume) was used as a carbon source after 120 h of incubation. NaNO3 increased xylanase produc...

متن کامل

Xylanase Production under Solid State Fermentation by Aspergillus niger

 Central composite orthogonal design was applied to quantify relations  of xylanase production, loss of dry matter and change of pH with four critical variables during solid state fermentation of a mixture of wheat bran and wheat straw on which Aspergillus niger CCUG 33991 was cultivated. The studied variables included the percentage of wheat straw, temperature, moisture content, and fermentati...

متن کامل

Polymorphism in the ITS region of ribosomal DNA of Cochliobolus sativus isolates differing in xylanase production.

The restriction of PCR-amplified internal transcribed spacers (ITS) ofribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular parameter used showed that C. sativus isolates reside in three phylogenetic groups. There was ob...

متن کامل

Optimization and Scale Up of Cellulase-Free Xylanase Production in Solid State Fermentation on Wheat Bran by Cellulosimicrobium sp. MTCC 10645

The production of cellulase-free xylanase was investigated with a locally isolated and identified strain, Cellulosimicrobium sp. MTCC 10645 in solid state fermentation. Different fermentation conditions were standardised for the growth and xylanase activity, the optimum being 72 h growth at pH 7.0, cultivation temperature 40°C and substrate to moisture ratio of 1:1.8 (w/v). Among different lign...

متن کامل

Production of an alkali tolerant extracellular xylanase from Bacillus pumilus VLK-1 in solid state fermentation and its use in tomato juice clarification

Xylanase production from Bacillus pumilus VLK-1 in solid state fermentation was enhanced through optimization of the process using one variable approach. The enzyme production was highest (42324±786 IU/g) upon 96h of incubation at 37°C using wheat bran as a substrate moistened with mineral salt solution (pH 9.0) containing 0.1% folic acid, 0.2% riboflavin and 6.0% yeast extract in the ratio of ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 39  شماره 

صفحات  -

تاریخ انتشار 2008